For other nucleic acid methods (RiboGreen, OliGreen), quantified in thePicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. 2022. The Quant-iT™ DNA Assays are designed for high throughput (>20 samples) and for use in 96-well plate readers with no further adaptation. (Waltham, MA, United States) using the Quant-iT TM PicoGreen TM dsDNA Assay Kit. Quantitation Range. Rapidly detect as little as 0. • Suitable for use in 96- and 384-well plate formats. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of ssDNA and RNA with minimal effect on the quantitation results. A 1:200 dilution of PicoGreen reagent is prepared by adding 10 μl of PicoGreen per 2 mL of 1X TE buffer. Triplicate 10 μL samples of λ DNA (Ο), E. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. PicoGreen dsDNA quantitation reagents are. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. no. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R11490Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). Description. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Rapidly detect as little as 0. The DeNovix dsDNA High. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Prepare the rest of the standards , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. 500/525. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. The Quant-iTTM PicoGreenTM dsDNA Reagent enables researchers to quantitate as little as 250 pg/mL of dsDNA (50 pg dsDNA in a 200 μL assay volume) with a fluorescence. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Material Amount Concentration Storage Stability Quant-iT™ PicoGreen® dsDNA reagent (Component A) 1 mL in 1 vial (P7589) or in10 vials of 100 μL each (P11496) Solution in DMSO 2–6ºC Desiccate Protect from light • • • When stored as directed, product stable. Quantify nucleic acids and proteins using four user-selectable fluorescent. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). with the Qubit dsDNA BR Assay Kit and 1 µL of each sample was measured in quadruplicate with the Quant-iT™ PicoGreen dsDNA Assay Kit. Quant-iT PicoGreen dsDNA Assay Kits and Reagents are designed to selectively detect dsDNA from a wide variety of sources, even in presence of ssDNA, RNA, and free. The Quant-iT™ dsDNA BR assay can be adapted for use in cuvettes or 384-well microplates. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo. n = 3. 2000 ng/mL of lamda dsDNA was serially diluted in 1XTE before the addition of the PicoGreen working solution. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267dsDNA in the presence of equimolar concentrations of ssDNA and RNA with minimal effect on the quantitative results. Bioz Stars score: 86/100, based on 1 PubMed citations. Bioz Stars score: 99/100, based on 1 PubMed citations. Equal amounts (100 µL) of working PicoGreen quantitation reagent were mixed and incubated for 10 minutes at room temperature, protected from light. Calibrator MPO concentration was quantified with a commercially available assay (Human Myeloperoxidase Quantikine ELISA Kit, Bio-Techne, Minneapolis, MN) according to the manufacturer’s recommendations. The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. • Suitable for use in 96- and 384-well plate formats. 双链DNA（dsDNA）定量检测试剂盒 【产品升级声明】： 我司（懋康生物）从22. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. Unit Size. Working PicoGreen reagent was prepared by diluting the concentrated DMSO-PicoGreen stock solution, provided in the PicoGreen kit, 1:200 with TE according to the kit instructions. • Specific for dsDNA in the presence of RNA. 4 mL of prewarmed to 37°C, TE Buffer Cat# D001 or other qualified TE buffer. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. No. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5 microplate reader. The differences in mechanical testing, cell proliferation in PrestoBlue™ and Quant-iT™ Picogreen™ dsDNA Assay were evaluated using t-test and one-way ANOVA, followed by Tukey’s post-test with α = 0. 2-200 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iTTM DNA Assay Kit, Broad Range 2-1000 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iT™ DNA Assay Kit, High Sensitivity 0. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. It requires the most user preparation and calculation, but can be adapted for cuvettes or plates. (An additional 1 ml TE with 5 ul PicoGreen may need to be prepared for the standards. Rapidly detect as little as 0. Quant-iT dsDNA BR buffer (Component B) 250 ml c. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Optimized for fluorescence microplate readers. The PG reagent from the Quant-iT TM PicoGreen TM dsDNA Assay Kit (Invitrogen) was prepared immediately before use by making a 1:200 dilution of the PG in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7. The AccuBlue® High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0. DNA standard curve. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 995 mL TE for the . Fluorescence was measured at. SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains—Table 9. 2. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. ZERO BIAS - scores, article reviews, protocol conditions and moreSelectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. Picogreen assays are compatible with most microplate readers and fluorometers. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Rapidly detect as little as 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. The dynamic range of this assay in microplate format, as stated in the product manual, is from 250 pg/mL to 1000 ng/mL with a single dye concentration. Catalog Number Specifications Unit Size Product Type Quantitation Range Packaging Type Price (EUR) Availability Quantity;The assay was prepared for 184 aliquots of DNA sample and five DNA standards, including one blank. • Suitable for use in 96- and 384-well plate formats. To perform fluorometric quantification of cfDNA quantity Quant-iT PicoGreen dsDNA Assay Kit (Thermofisher), was used, following the manufacturer’s instructions. Samples were excited at 480 nm and the fluorescence was measured at 520 nm using a TECAN Spark multimode reader. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. 99. 9, 10 The Pico-Green assay is sensitive to 25 pg dsDNA/mL, which is 400-fold more sensitive than Hoechst 33258 dye. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. Quant-iT PicoGreen dsDNA Assay Kit. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Picogreen assays are compatible with most microplate readers and fluorometers. Both fluorescent assays were performed with a Qubit fluorometer. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double. Product Type. High tolerance for contaminants. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Short-read sequencing was performed on an Illumina NextSeq 2000 platform (2x151bp. Bioz Stars score: 86/100, based on 1 PubMed citations. 実験してみた – PicoGreenをプレートリーダーで使用してみた. ” J Pharm Biomed Anal. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). Picogreen assay kit (Invitrogen, P7589) a. • Specific for dsDNA in the presence of RNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. One microgram of DNA was sheared using an M220. 2. 参考資料. Le réactif et les kits de quantification d’ADNdb PicoGreen sont parfaits pour les dosages PCR, les échantillons de biopuces, les dosages de lésions de l’ADN, les dosages d’activité enzymatique, la quantification d’ADN génomique, la mesure de l’ADNdb dans des mélanges complexes et la quantification d’ADN viral. Figure 2. Because the PicoGreen dye is an extremely sensitive detection reagent for dsDNA, it is imperative that the TE buffer is free of contaminating nucleic acids. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. quant it picogreen dsdna assay kit , 2023-10. Specifications Documents Scientific Resources Application Notes Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. ZERO BIAS - scores, article reviews, protocol conditions and moreGot it. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was added to 199 μl of a Qubit working solution. Tris-EDTA Buffer (10 mM tris, 1 mM EDTA), pH8. For highly selective detection of dsDNA over RNA, even in the presence of contaminants. 2–100 ng, the fluorescence signal is linear with DNA. The assay is linear over three orders of magnitude and has little sequence. 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. Rapidly detect as little as 0. Highly selective for dsDNA over ssDNA or RNA. Rapidly detect as little as 0. Rapidly detect as little as 0. P7589) was used to quantify DNA by fluorescence. LC3, and dsDNA, or α-synuclein, cathepsin D and dsDNA, Picogreen (1/10000, Quant-iT PicoGreen dsDNA Assay Kit. 99. Prepare only the amount of 1:25 diluted PicoGreen required for that run. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. 995 mL TE for the . The assay is linear over three orders of magnitude and has little sequence dependence. 3. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. to each well. 5 µL of each extracted sample was used for Picogreen analysis. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 5 ng/mL 990 10 1000 250 pg/mL 999 1 1000 25 pg/mL 1000 0 1000 Blank Figure 1. In addition to conventional determination using 5 standards as present-ed by the manufacturer, we will show that reliable determi-nation is possible using only 2 standards. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). DNA was quantified by the Quant-it PicoGreen dsDNA Assay Kit (Invitrogen Life technologies, UK) according to the manufacturer’s protocol. For a standard. Skip to main list 美谷分子仪器（上海）有限公司. Cloning, expression and proteins purification. 0 fluorometer according to the manufacturer’s protocols; a. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of. 3 Q32854 26. 2 - 100 ng/ml 480/520 NaCl, MgCl 2Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. This protocol is a simplified and c. ZERO BIAS . 10pg/µl vs. The assays are many times more sensitive than conventional UV absorbance and can be optimized for different concentration ranges. The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). Picogreen 定量试剂盒兼容大多数微孔板读数仪和荧光计。. Final libraries were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a SpectraMax iD3 (Molecular Devices). In addition, the concentration of DNA obtained from the 1860 tomato samples was measured fluorometrically using Quant-iT™ PicoGreen™ dsDNA Assay Kit (hereafter PicoGreen, Thermo Scientific, Waltham, MA, USA) and a 96-wells plate reader VICTOR3 1420 (PerkinElmer, Waltham, MA, USA) equipped with an excitation filter F485 and. 3 Q33120 39. An Agilent 2100 Bioanalyzer (Agilent) was used to compare library quality using a High Sensitivity DNA Kit (Agilent), and the purified library was quantified using a Quant-iT PicoGreen dsDNA assay kit (Invitrogen). 21 DiB) [22]. 5. Quantitation Range. Using this data as a starting point, the optimal conditions (e. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. ) Add PicoGreen to DNA samples Add 100 1x PicoGreen to each well of the DNA/standard plate without contaminating tips. Using 20x TE buffer make 1x solution. 99. PicoGreen® Assay Supplies Equipment: − NanoDrop 3300 Fluorospectrometer − 2uL pipettor (low retention nuclease free pipette tips) Materials:Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. Picogreen assays are compatible with most microplate readers and fluorometers. The Rapidly detect as little as 0. Qubit® dsDNA BR Assay Kit. Selection guide for the Qubit and Quant-iT Assay Kits—Table 8. For Research Use Only. 2. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). 10 ng/mkl – 10000 ng/ml. The PicoGreen dsDNA quantitation assay can be used when selectively detecting as little as 25 pg/ml of dsDNA with free nucleotides, RNA and ssDNA present, having minimal impact on the intensity of. Louis, MI, USA). PicoGreen dsDNA quantitation reagents are. Regulations & Guidance; Manuals 0 0. Begin typing to search, use arrow keys to navigate, Enter to select. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Protocols All Picogreen reactions and measurements werethe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. 2023-11. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation. 実験してみた – PicoGreenをプレートリーダーで使用してみた. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Description. No. For example, if the amount of the linear dsDNA library available is 20 ng, the linear dsDNA library input should be 10 ng, and the linear dsDNA library concentration should be at least 0. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R114909. Natural cures with herbs are. Quant It Picogreen Dsdna Quantitation Assay Kit, supplied by Thermo Fisher, used in various techniques. The kit contains. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. dsDNA Assay Kit: Invitrogen Quant-iT PicoGreen dsDNA Assay Kits and dsDNA Reagents, spectrophotometer, qPCR Quantification Kit: Roche KAPA Library Quantification Kits — Complete Kit (Universal), and qPCR Plate Reader: LightCycler 480 Instrument II. 1X dsDNA assay (%) 100 17. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. 1 Warm Quant-iT PicoGreen reagent to room temp in the dark. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. 2 – 100 ng. The original sequencing data. The assay was prepared for 184 aliquots of DNA sample and five DNA standards, including one blank. According to the obtained results, the assay presents a linear correlation in a wide range of 0. Three kits covering a wide range of dsDNA concentrations. Quantitation Range. 5mL. Quant-iT PicoGreen dsDNA Assay Kit a. Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). VIEWS. Revised: 10–June–2008 | MP 07581 Table 1. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Helixyte™ Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. 5 – 1,000 ng/ mL DNA with a high dynamic range (Fig. Catalog Number. Natural cures with herbs are. 50 pg to 2 µg. coli rRNA (Δ), or a 1:1 mixture of DNA and RNA (∎) were assayed in the Quant-iTTM dsDNA HS assay. ZERO BIAS - scores, article reviews, protocol conditions and moreAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Mix by gently vortexing for 5 seconds. 5 ng/ml of dsDNA were detected with minimal effect in the presence of ssDNA, RNA, and free nucleotides, which is comparable with InvitrogenTM Quant-iT™ PicoGreen® dsDNA Reagent. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Generation of a standard curve using dsDNA-Standards Preparation of the measurement solution was based on the user manual of the Quant-iT, PicoGreen dsDNA kit. No. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. The user guide provides detailed information on the product description, component concentration, storage, assay protocol, and safety data sheets of the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit. Quantitation Range. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Picogreen assays are compatible with most microplate readers and fluorometers. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. 7 Q33232 4 Green features Less waste and use of fewer resources With the Qubit and Quant-iT 1X dsDNA HS and 1X dsDNA BR Assay Kits, instead of supplying the assay reagent and dilution buffer in separate bottles as was done with the. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). 2–100 ng, the fluorescence signal is linear with DNA. When 5–500 pg salmon dsDNA or 3–1000 pg control dsDNA (calf thymus dsDNA standard) is added to AccuClear a linear response is observed (Fig. Quantitation Range. Selectively detect dsDNA from a wide variety of sources, and even in presence of. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. Bioz Stars score: 86/100, based on 1 PubMed citations. Blog May 11, 2021. Add-and-read format makes measuring low concentrations of dsDNA simple—no dilutions, no extra tubes. Accuracy of the Qubit dsDNA HS assay compared to the Quant-iT PicoGreen dsDNA assay plotted on two different types of curves. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. Unit Size. Sample fluorescence was. Showing 4 of 4. The Quant-iT™ OliGreen™ ssDNA Assay Reagent enables researchers to quantify as little as 1 ng/mL oligonucleotide or ssDNA (200 pg in a 200 µL assay volume) with a. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. • Suitable for use in 96- and 384-well plate formats. 2 to 2,000 ng/µL, providing a detection range of 4−2,000 ng. Unit Size. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. 5 µL of each extracted sample was used for Picogreen analysis. 2mL 1 x 96 wellsAssay Plate with Plate Sealing Foil, D002 *Component can be purchased separately. The reagent Quant-iT PicoGreen was added to all wells and the samples were incubated for 5 min at room. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Rapidly detect as little as 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The PG reagent from the Quant-iT TM PicoGreen TM dsDNA Assay Kit (Invitrogen) was prepared immediately before use by making a 1:200 dilution of the PG in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7. The assay is performed at room temperature, and the signal is stable for 3 hours. UV흡광 결과보다 민감도 우수. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. PicoGreen dsDNA quantitation reagents are. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. 9 Q33230 9 500 28. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). The Molecular Probes PicoGreen®. The following protocol is an adaptation of the Mo-lecular ProbesTM dsDNA Quantitation Reagent and Kits product information sheet. Picogreen assays are compatible with most microplate readers and fluorometers. stranded DNA (dsDNA). • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 0 mL to 200 uL to fit a 96-well microplate format. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM製品番号（カタログ番号）： P11495. 20 and 2. Pico Green dsDNA Assay 2 03/04/2019The assay was performed according to the initial MPO-DNA complex ELISA protocol, but blocking buffer, sample diluent and detection antibody diluent were replaced by an in-house blocking buffer. Quant-iT PicoGreen dsDNA kit by Thermo Scientific Catalog #: P7589 2Prepare 1XTE buffer from 20X stock solution using nuclease-free water: will need 200 μl/well (for. high-range. 2023. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. The stock DNA and the corresponding 1:5 diluted DNA derived from FFPE tissue was quantified by using Quant-iT™ PicoGreen dsDNA Assay kit (Thermo Fisher Scientific) as follows: 2 μl of sample DNA were diluted 1:5 vol/vol in 1× TE, containing 1:100 diluted PicoGreen reagent in a final reaction volume of 40 μl per reaction. 7. PicoGreen dsDNA quantitation reagents are. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. 99 to $1999. PicoGreen Solution must be diluted fresh before each assay run. 3,201 3. Fluorescence signal measured for the DNA sodium salt standard from calf thymus using the Quant-iT™ PicoGreen® assay. 2–100 ng: 2-1,000 ng: 0. . For example, if the assay requires 50 wells, add 600 L of PicoGreen to 14. ITSkabir). In case dsDNA standards are available only the quantification reagent can be used. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. Unit Size. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. no. Picogreen assays are compatible with most microplate readers and fluorometers. 5 mm in the Eppendorf BioSpectrometer fluorescence, PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. USA) and quantity was measured using Qubit 2. Rapidly detect as little as 0. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 5). Optimized for fluorescence microplate readers. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Suitable for use in 96- and 384-well plate formats. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Fluorometric dsDNA Assay. The Quantus™ NGS Starter package is cost-effective and easy to incorporate into your laboratory. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Mit dem PicoGreen dsDNA. Plasma levels. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. P7589. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. 2. Picogreen assays are compatible with most microplate readers and fluorometers. dsDNA and PicoGreen analyzed using the Modulus and the Blue Fluorescence Optical Kit. Quant-iT PicoGreen dsDNA Assay Kit. 0). A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. All NGS libraries generated with both the Fragmentase and the FTP method contained similar amounts of ds DNA (800 ± 50 ng) and had similar mean insert sizes of the libraries in a range from 400 to 500 bp. Breadcrumb. In this experiment we followed the instructions of the Quant-iT™ PicoGreen dsDNA Assay Kit to compare setup on the Matrix Hydra DT and Matrix PlateMate 2x2 to manual setup and then reduced the total volume from 210 µl to 105 µl on both instruments. 2 DNA selectivity and sensitivity of the Quant-iT dsDNA high-sensitivity assay. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. Not for use in diagnostic procedures. PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. 125 to 100 ng/mL. Compared with the aforementioned methods, the PicoGreen dye binding assay for dsDNA has greater sensitivity, specificity, and linear range. 2. A12216) Metabolic (glucose) • Amplex Red Glucose/Glucose Oxidase Assay Kit (Cat. Sequencing was performed with a HiSeq Single-End Illumina platform (USA) [Citation 31]. Effect of the buffer dilution on the DNA measurement with the Quant-It PicoGreen dsDNA assay. 2 to 100 ng) Assay Kit were used with a Qubit 3. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. The reagent must be completely thawed before using it by bringing it to room temperature. DNA standard curve. Qubit assays are available for double- and single-stranded DNA (dsDNA and ssDNA), RNA, microRNA, and protein. high-range. After the reagent thawed, it is advisable to briefly vortex the tube toPlasma levels of cfDNA were measured using a fluorometric assay for double-stranded DNA Quant-iT PicoGreen dsDNA Assay kit (Invitrogen) following the manufacturer’s instructions. 作成者 LatB Staff 01. yields in a method for direct cycle sequencing of PCR products. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Coleen - 06 Oct. Thermo Fisher quant it picogreen dsdna quantification reagent kit Quant It Picogreen Dsdna Quantification Reagent Kit, supplied by Thermo Fisher, used in various techniques. . In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. 2. 7 Q33231 6 1,000 41. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 2. using a PicoGreen assay by the company life technologies (Quant-iT™ PicoGreen dsDNA Reagent and Kits). Control samples were not lysed and consisted of free pDNA. All LNP samples were diluted 300-fold in TE buffer and placed on a Corning ® 96 Well Solid Polystyrene Microplate (Sigma-Aldrich, St. Quant-iT OliGreen ssDNA Assay Kits contain an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Quant-iT Assay Kits Quant-iT DNA Assay Kit, High Sensitivity The Quant-iT High-Sensitivity DNA Assay Kit has a linear detection range of 0. low-range. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 0 ml 200x in DMSO b. Figure 8. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 99. quant it picogreen dsdna quantitation assay kit - by Bioz Stars , 2023-09. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec.